Author Affiliations
Abstract
1 School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
2 College of Science, Shanghai Institute of Technology, Shanghai 201418, China
3 Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen 361102, China
We develop an improved region growing method to realize automatic retinal pigment epithelium (RPE) cell segmentation for photoacoustic microscopy (PAM) imaging. The minimum bounding rectangle of the segmented region is used in this method to dynamically update the growing threshold for optimal segmentation. Phantom images and PAM imaging results of normal porcine RPE are applied to demonstrate the effectiveness of the segmentation. The method realizes accurate segmentation of RPE cells and also provides the basis for quantitative analysis of cell features such as cell area and component content, which can have potential applications in studying RPE cell functions for PAM imaging.
110.5120 Photoacoutic imaging 110.0180 Microscopy 100.2000 Digital image processing 170.1530 Cell analysis Chinese Optics Letters
2017, 15(5): 051101
Author Affiliations
Abstract
Institute for Laser Medicine and Bio-Photonics, Department of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240
The emergent light distribution of a new type of contact laser scalpel is measured in three different states using a light sensor. The relationship between the angle and the light intensity is analyzed. The results show that the strongest light is emitted from two sides and the front of the scalpel. The light from the front mainly plays a role of cutting. The light from two sides contributes to stanch the wound so as to remain a clear visual field during the surgery. It also helps to increase the cutting efficiency.
激光刀头 出射光分布 绿激光 激光手术 170.0170 Medical optics and biotechnology 170.1610 Clinical applications 140.3480 Lasers, diode-pumped Chinese Optics Letters
2008, 6(12): 913
